Human Connective Tissue Growth Factor, CTGF ELISA Kit CKERS-CTGF-007H from Creative Diagnostics

Product Specs
ItemHuman Connective Tissue Growth Factor, CTGF ELISA Kit
CompanyCreative Diagnostics
Price Supplier Page View Company Product Page
Catalog NumberCKERS-CTGF-007H
Quantity10 plates
TemperatureCapture Antibody: 100µg of antigen-affinity purified goat anti-CTGF. Centrifuge vial prior to opening. Reconstitute in 1.0 ml sterile water for a concentration of 100µg/ml. Following reconstitution the Capture antibodies may be stored at 2-8°C for up to 6 months. For long term storage it is recommended to aliquot into working volumes and store at -70°C in a manual defrost freezer.
Detection Antibody: 50µg of biotinylated antigen-affinity purified goat anti-CTGF. Centrifuge vial prior to opening. Reconstitute in 0.5 ml sterile water for a concentration of 100µg/ml. Following reconstitution the Detection antibodies may be stored at 2-8°C for up to 6 months. For long term storage it is recommended to aliquot into working volumes and store at -70°C in a manual defrost freezer.
Human CTGF Standard: 1 µg of recombinant CTGF. Centrifuge vial prior to opening. Reconstitute in 1.0 ml sterile water for a concentration of 1 µg/ml. The Standard may be stored at 2-8°C for one month or aliquoted and stored at -70°C for up to three months in a manual defrost freezer.
UltraAvidin-HRP Conjugate: 40µl vial. Upon receipt, UltraAvidin-HRP conjugate should be stored at 2-8°C, DO NOT FREEZE.
TMB Liquid Substrate: Aspirate and wash plate 4 times. Add 100µl of TMB HRPO Microwell Substrate Standard Kinetic One Component "Ready Use" to each well. Incubate at room temperature and monitorcolor development with an ELISA plate reader at 450 nm with wavelength correction set at 540 nm or 570 nm. The reaction may be stopped after 15-20 minutes by adding 100µl of 2 M sulfuric acid to each well
ApplicationsELISA
ReactivityHuman
ComponentsCapture Antibody: 100 µg of antigen-affinity purified goat anti-CTGF. Centrifuge vial prior to opening. Reconstitute in 1.0 ml sterile water for a concentration of 100 µg/ml. Following reconstitution the Capture antibodies may be stored at 2-8°C for up to 6 months. For long term storage it is recommended to aliquot into working volumes and store at -70°C in a manual defrost freezer. Avoid repeated freeze and thaw cycles.
Detection Antibody: 50 µg of biotinylated antigen-affinity purified goat anti-CTGF. Centrifuge vial prior to opening. Reconstitute in 0.5 ml sterile water for a concentration of 100 µg/ml. Following reconstitution the Detection antibodies may be stored at 2-8°C for up to 6 months. For long term storage it is recommended to aliquot into working volumes and store at -70°C in a manual defrost freezer. Avoid repeated freeze and thaw cycles.
Human CTGF Standard: 1 µg of recombinant CTGF. Centrifuge vial prior to opening. Reconstitute in 1.0 ml sterile water for a concentration of 1 µg/ml. The Standard may be stored at 2-8°C for one month or aliquoted and stored at -70°C for up to three months in a manual defrost freezer. Avoid repeated freeze and thaw cycles.
UltraAvidin-HRP Conjugate: 40 µl vial. Upon receipt, UltraAvidin-HRP conjugate should be stored at 2-8°C, DO NOT FREEZE.
TMB Liquid Substrate:
(TMB Substrate should be at ambient temperature prior to use)
Aspirate and wash plate 4 times. Add 100 µl of TMB HRPO Microwell Substrate Standard Kinetic One Component "Ready Use" to each well. Incubate at room temperature and monitor color development with an ELISA plate reader at 450 nm with wavelength correction set at 540 nm or 570nm. The reaction may be stopped after 15-20 minutes by adding 100 µl of 2 M sulfuric acid to each well
NCBI Full Gene Namecellular communication network factor 2
NCBI Gene AliasesIGFBP8, CTGF, HCS24, NOV2

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Creative Diagnostics

45-1 Ramsey Road

Shirley, N 11967

United States

Phone: 1-631-624-4882 (USA)
44-161-818-6441 (Europe)

Email: info@creative-diagnostics.com

Company Profile

Website: https://www.creative-diagnostics.com/

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Bench Tips for Optimizing ELISA: It All Comes Out in the Wash
August 22, 2013
This article will provide you with tips on washing your ELISA plates using an automated plate washer, with the goal of avoiding high background and high variation (between plates and between replicate wells). read more

Human Connective Tissue Growth Factor, CTGF ELISA Kit from Creative Diagnostics

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Creative Diagnostics

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Bench Tips for Optimizing ELISA: It All Comes Out in the Wash

Human Connective Tissue Growth Factor, CTGF ELISA Kit from Creative Diagnostics

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